Catalog Number - 85018-15P

The 85018‑15P Cogent RP C18 ABX™ HPLC Column is engineered for high‑resolution, scalable chromatography, delivering reliable performance across a wide pH range (2–12).

Constructed with a bulky, specially end‑capped C18 phase bonded to high‑purity silica, this column minimizes unwanted interactions while enhancing peak shape for acidic, basic, hydrophobic, and neutral compounds, including analytes with extreme pKa values.

✅ Ideal as a USP L1 alternate for demanding acid/base methods

150 mm Length – Maximum Resolution Before Scale‑Up

• Higher resolving power than 50 mm and 100 mm columns

• Improves separation of closely related compounds

• Increases peak capacity for complex mixtures

• Ideal for method optimization prior to preparative scale‑up

✅ Selected when maximum resolution is required before transitioning to preparative workflows

4.6 mm ID – Analytical to Preparative Bridge Platform

• Compatible with standard HPLC systems

• Ideal for:

  • method development and refinement

  • scale‑up planning

  • analytical validation work

Typical Flow Rate Range

• ~0.8–1.5 mL/min (supports higher flows due to 10 µm particle size)

✅ Serves as a direct bridge between analytical and preparative chromatography

10 µm Particle – Preparative Scale‑Up & High‑Flow Performance

• Designed for:

  • High flow rates

  • Low backpressure operation

  • Durability under high sample loads

✅ Ideal for:

• Preparative method development

• Process scale‑up workflows

• Systems with pressure limitations

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Supports:

  • Highly acidic mobile phases

  • Strongly basic mobile phases

• Suitable for:

  • Strong acids

  • Strong bases

✅ Enables separations not possible with standard C18 columns

Why Choose RP C18 ABX™ Columns (10 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for challenging analytes and aggressive conditions

Preparative Scalability

• Designed for method transfer to preparative columns

• Ensures consistent scale‑up performance

Low Backpressure Advantage

• Enables higher flow rates with reduced system strain

• Compatible with standard HPLC systems

✅ Preferred for method development where scalability and robustness are critical

Application Performance Advantages

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

• Enables:

  • Reliable separations in high‑flow environments

  • Consistent performance under extreme pH conditions

✅ Ideal for process development and preparative method optimization

System & Sample Considerations

• Compatible with standard HPLC systems

• Suitable for high‑flow analytical and preparative‑leaning workflows

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column fouling

• Maintain long‑term performance and reproducibility

Typical Applications

• Preparative method development and scale‑up

• HPLC method optimization

• Acidic and basic compound separations

• Pharmaceutical process development

• Stability‑indicating methods

• High‑flow analytical workflows

Important Usage Notes

• Designed for standard HPLC systems (4.6 mm ID)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for maximum‑resolution method development prior to scale‑up

• Optimize flow rate based on throughput and resolution requirements